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Furosemide was devoid of mutagenic activity in various strains of Salmonella typhimurium when tested in the presence or absence of an in vitro metabolic activation systemand questionably positive for gene mutation in mouse lymphoma cells in the presence of rat liver S9 at the highest dose testedFurosemide did not induce sister chromatid exchange in human cells in vitrobut other studies on chromosomal aberrations in human cells in vitro gave conflicting resultsIn Chinese hamster cells it induced chromosomal damage but was questionably positive for sister chromatid exchangeStudies on the induction by furosemide of chromosomal aberrations in mice were inconclusiveThe urine of rats treated with this drug did not induce gene conversion in Saccharomyces cerevisiae
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